Monomer. Homodimer. Interacts with FGFR1, FGFR2, FGFR3 and FGFR4. Affinity between fibroblast growth factors (FGFs) and their receptors is increased by heparan sulfate glycosaminoglycans that function as coreceptors. Interacts with CSPG4, FGFBP1 and TEC. Found in a complex with FGFBP1, FGF1 and FGF2.
Function:
Plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. Functions as potent mitogen in vitro.
Subcellular Location:
Secreted
Exported from cells by an endoplasmic reticulum (ER)/Golgi-independent mechanism. Unconventional secretion of FGF2 occurs by direct translocation across the plasma membrane.
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s
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Precision
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Intra-assay Precision (Precision within an assay):Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Intra-Assay CV: ≤4.9%
Inter-assay Precision (Precision between assays):Three samples of known concentration were tested in five separate assays to assess inter-assay precision.
Inter-Assay CV: ≤9.8%
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Recovery
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Recovery was determined by spiking various levels of FGF2 into serum and plasma .
Sample Type
Average(%)
Recovery Range(%)
Serum
97
92-100
Plasma
98
96-107
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Linearity
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The linearity of the kit was assayed by testing samples spiked with appropriate concentration of FGF2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
serum(n=5)
93-102%
94-101%
92-104%
90-103%
EDTA plasma(n=5)
94-103%
92-102%
94-102%
91-107%
heparin plasma(n=5)
92-104%
90-107%
93-104%
95-102%
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